Test Performance Studies

Performance Studies by Application


 
Staphylococci
Deck MK, Anderson ES, Buckner RJ, Colasante G, Coull JM, Crystal B, Della Latta P, Fuchs M, Fuller D, Harris W, Hazen K, Klimas LL, Lindao D, Meltzer MC, Morgan M, Shepard J, Stevens S, Wu F, Fiandaca MJ.
Multicenter Evaluation of the Staphylococcus QuickFISH Method for Simultaneous Identification of Staphylococcus aureus and Coagulase Negative Staphylococci Directly from Blood Culture Bottles in less than Thirty Minutes.  J Clin Microbiol. 2012 Apr 4. [Epub ahead of print].
 
F. Wu*, S. Whittier, M. Fiandaca, B. Crystal, P. Della-Latta
20-minute identification of enterococci and Staphylococcus aureus causing bloodstream infections by QuickFISH™, a novel assay based on PNA technology.  Poster # P-2373. ECCMID 2012.

Lene Leerbeck, Dennis Schroder Hansen Department of Clinical Microbiology, Hillerod Hospital Denmark  
Rapid QuickFISH compared to standard PNA FISH for identifcation of S. aureus and CNS. Poster # P-1499. ECCMID 2012.   
 
E. Carretto, M. Mirra, M. Bardaro, S. Ferretti, C. Zuelli  
Evaluation of the QuickFISH BC test, A rapid method to distinguish Staphylococcus aureus from coagulase-negative staphylocococci in positive blood cultures. Poster. ECCMID 2012.   
 
Benjamin Parcell, Gillian Orange
Evaluation of Peptide Nucleic Acid Fluorescence In Situ Hybridization (PNA FISH) Assay for the Identification of Gram-Positive Cocci (GPC) and Gram-Negative Bacilli (GNB) from Blood Cultures. Poster. FIS 2011.
 
T. M. Painter, P. R. Lephart, R. Mitchell, M. R. Fairfax, H. Salimnia
Thirteen Months' Experience with the Rapid PNA FISH® Protocol for Identification of Staphylococcus aureus from Positive Blood Culture Bottles. Poster # C-1113. ASM 2010.
 
Hensley DM, Tapia R, Encina Y. 
An evaluation of the AdvanDx Staphylococcus aureus/CNS PNA FISH assay. Clin Lab Sci. 2009 Winter;22(1):30-3.
 
Morgan M, Deirboghossian A, Youssef E. 
Rapid PNA FISH Protocol Modification for Detection of Staphylococci. Poster. ASM 2008.
 
Novak-Weekley SM, LaForga M, Carey L, Frazier L. 
Validation of S. aureus PNA FISH, E. faecalis PNA FISH & C. albicans PNA FISH for Rapid Identification of Positive Blood Culture Bottles. Poster # C-007. ASM 2006. 
 
Wilson DA, Hall GS, Procop GW. 
Evaluation of the Staphylococcus aureus/Coagulase Negative Staphylococci dual-labeled PNA FISH Probe on Positive Blood Cultures with Graph Positive Cocci in Clusters. Poster # C-060. ASM 2006.
 
Fiandaca M, Shepard J, Strauss S, Casiano-Colon A, Romansky S, Hulbert B, Schultz S, Kuusela P, Ristow K, Janda W, Cioffi F, Danielson N, Harkins T, Johansen J. 
A Single-Blinded Multicenter Evaluation of Three PNA FISH Kits for Rapid Species Identification Directly from Blood Culture Bottles. Poster # C-268. ASM 2006.
 
Vogel SA, Warner DK, Schindler SA, Hall GS, Procop GW. 
FISHing in the Trenches: Performance of the S. aureus PNA FISH Test on the Routine Blood Culture Bench. Poster # C-058. ASM 2006.
 
V. Whitehead, T. Castellone, K.C. Chapin, G. Colasante, D.G. Beckwith, M. Gherna, P.D. Stamper, K.C. Carroll, K.M. Olivera, W. Gillespie, T. Profft Larsen, L. Horvat, J.R. Shepard, Mark Fiandaca 
Evaluation of Dual Color S. aureus/CNS PNA FISH for Simultaneous Identification of Staphylococcus aureus and Coagulase-Negative Staphylococci Directly from Positive Blood Culture Bottles. Poster # C-239. ASM 2007.
 
González V, Padilla E, Giménez M, Vilaplana C, Pérez A, Fernández G, Quesada MD, Pallarés MA, Ausina V. 
Rapid diagnosis of Staphylococcus aureus bacteremia using S. aureus PNA FISH. Eur J Clin Microbiol Infect Dis. 2004 May;23(5):396-8. Epub 2004 Apr 27.
 
Rausei V, Youssef E, Morgan M. 
Direct identification of Staphylococcus aureus, Enterococcus faecalis, and Candida albicans from blood cultures using fluorescence in situ hybridization with nucleic acid probes. Poster # V-002. ASM 2004.
 
 
Oliveira K, Brecher SM, Durbin A, Shapiro DS, Schwartz DR, De Girolami PC, Dakos J, Procop GW, Wilson D, Hanna CS, Haase G, Peltroche-Llacsahuanga H, Chapin KC, Musgnug MC, Levi MH, Shoemaker C, Stender H. 
Direct identification of Staphylococcus aureus from positive blood culture bottles. J Clin Microbiol. 2003 Feb;41(2):889-91. 
 
Oliveira K, Procop GW, Wilson D, Coull J, Stender H. 
Rapid identification of Staphylococcus aureus directly from blood cultures by fluorescence in situ hybridization with peptide nucleic acid probes. J Clin Microbiol. 2002 Jan;40(1):247-51. 


Enterococci
F. Wu*, S. Whittier, M. Fiandaca, B. Crystal, P. Della-Latta
20-minute identification of enterococci and Staphylococcus aureus causing bloodstream infections by QuickFISH™, a novel assay based on PNA technology.  Poster # P-2373. ECCMID 2012.
 		 
   
M. Morgan, E.M. Marlowe, S.M. Novak-Weekley, J.M. Miller, T.M. Painter, H. Salimnia, B. Crystal
A New 1.5 Hour Procedure for PNA FISH for Identification of Enterococcus Species from Blood Cultures. Poster # C-1164. ASM 2010.
 
Gherna MR, Wakefield TH, Carroll KC. 
A Rapid, PNA FISH Protocol for the Direct Identification of Enterococcus faecalis and Other Enterococcus species Simultaneously from Positive Blood Bottles in Less than 1.5 hr. Poster # C-094. ASM 2008.
 
Novak-Weekley SM, LaForga M, Carey L, Frazier L. 
Validation of S. aureus PNA FISH, E. faecalis PNA FISH & C. albicans PNA FISH for Rapid Identification of Positive Blood Culture Bottles. Poster # C-007. ASM 2006. 
 
Fiandaca M, Shepard J, Strauss S, Casiano-Colon A, Romansky S, Hulbert B, Schultz S, Kuusela P, Ristow K, Janda W, Cioffi F, Danielson N, Harkins T, Johansen J. 
A Single-Blinded Multicenter Evaluation of Three PNA FISH Kits for Rapid Species Identification Directly from Blood Culture Bottles. Poster # C-268. ASM 2006. 
 
Johnson JK, Roberts AA, Forrest GN, Lincalis DP, Venezia RA. 
Rapid Identification of Enterococcus Species in Positive Blood Cultures with Therapeutic Implications. Poster # C-147. ASM 2006. 
 
Rausei V, Youssef E, Morgan M. 
Direct identification of Staphylococcus aureus, Enterococcus faecalis, and Candida albicans from blood cultures using fluorescence in situ hybridization with nucleic acid probes. Poster # V-002. ASM 2004.
 
Chapin KC, Musgnug MC, Oliveira K, De Girolami KC, Dakos J, Johansen JT, Procop GW, Wilson D, Padilla E, Gonzalez V, Stender H. 
Multicenter Evaluation of E. faecalis PNA FISH for Rapid Identification and Differentiation of Enterococcus faecalis and other Enterococcus species Directly from Positive Blood Cultures. Poster. ASM 2003.

 
Gram-Negatives
Benjamin Parcell, Gillian Orange
Evaluation of Peptide Nucleic Acid Fluorescence In Situ Hybridization (PNA FISH) Assay for the Identification of Gram-Positive Cocci (GPC) and Gram-Negative Bacilli (GNB) from Blood Cultures. Poster. FIS 2011.
 
P. Della-Latta, H. Salimnia, T. Painter, F. Wu, G.W. Procop, D.A. Wilson, W. Gillespie, A. Mender, B.S. Crystal
Identification of Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa in Blood Cultures: a Multicenter Performance Evaluation of a Three-Color Peptide Nucleic Acid Fluorescence In Situ Hybridization Assay. J Clin Microbiol.  2011 Jun;49(6):2259-61.
 
L. Leerbeck, D.S. Hansen
Evaluation of the new GNR Traffic Light PNA FISH fluorescence in situ hybridisation probes for rapid same-day identification of Gram-negative rods from positive blood culture bottles. Poster # P-2442. ECCMID 2011.
 
Raglio, C. Venturelli, D. Barbarini, A. Grosini, E. Carretto, P. Marone, F. Rumpianesi, P.A. Serna Ortega, M. Passera, A. Goglio 
Multicenter Evaluation of PNA FISH Test for Species Identification of Selected Gram Negative Bacilli, Gram Positive Cocci and Yeasts from Blood Cultures. Poster # P-1919. ECCMID 2011.
 
P. Della-Latta, H. Salimnia, T. Painter, F. Wu, G.W. Procop, D.A. Wilson, W. Gillespie, A. Mender, B.S. Crystal
Rapid Identification of Gram Negative Rods (Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae) from Positive Blood Culture Bottles by PNA FISH. Poster # 1596. ASM 2011.
 
A. Mender, S. Stevens, A. Mortensen, W. Gillespie, K. Oliveira, H. Stender, M. Fiandaca 
Evaluation of GNR Traffic Light PNA FISH for Identification of E. coli, K. pneumoniae and P. aeruginosa Directly from Positive Blood Culture Bottles. Poster # C-1586. ASM 2010.
 
Morgan M, Marlowe E, Della-Latta P, Salimnia H, Novak-Weekley S, Wu F, Crystal BS.
Multicenter evaluation of a new shortened peptide nucleic acid fluorescence in situ hybridization procedure for species identification of select Gram-negative bacilli from blood cultures. J Clin Microbiol.  2010 Jun;48(6):2268-70.
 
Søgaard M, Hansen DS, Fiandaca MJ, Stender H, Schønheyder HC. 
Peptide nucleic acid fluorescence in situ hybridization for rapid detection of Klebsiella pneumoniae from positive blood cultures. J Med Microbiol. 2007 Jul;56(Pt 7):914-7.
 



Candida
L. Hall, K.M. Le Febre, S.M. Deml, S.L. Wohlfiel, N.L. Wengenack
Evaluation of the Yeast Traffic Light(R) PNA FISH; Probes for Identification of Candida species from Positive Blood Cultures. J Clin Microbiol. 2012 Jan 11. [Epub ahead of print]
 
S. van den Tempel, V. Ozenci, L. Klingspor, M. Ullberg 
Rapid identification of yeasts by PNA FISH in patients with candidemia. Poster. ECCMID 2011.
 
C. Farina, S. Andreoni, C. Bonetti, P. Casella, M. Conte, P. Fazii, G. Lombardi, F. Luzzaro, E. Manso, P. Marone, C. Morazzoni, M. Passera, S. Perin, A. Rocchetti, S. Sanna , E.F. Vigano 
Evaluation of the PNA FISH technology for yeast identification directly from positive blood cultures. An Italian experience. Poster. ECCMID 2011.
 
D.A. Wilson, D.K. Warner, G.S. Hall, G.W. Procop 
Evaluation of the Rapid Yeast PNA FISH Protocol for Blood Cultures. Poster # C-1108. ASM 2010.
 
E.M. Marlowe, M. Morgan, S.M. Novak-Weekley, J.M. Miller, T.M. Painter, H. Salimnia, B. Crystal
Multicenter Assessment of a Rapid PNA FISH Method for the Identification of Candida Species from Blood Culture Bottles. Poster # C-2073. ASM 2010.
 
L. Hall, K.M., S.M. Deml, S.L. Wohlfiel, N.L. Wengenack
PNA FISH Traffic Light Probes for Identification of Candida species from Blood Cultures. Poster # C-2068. ASM 2010.
 
W.G. Merz, M. Gherna 
A Rapid PNA FISH Protocol for the Direct Species-Specific Identification of Candida species From Positive Blood Bottles in Less than 1.5 hr. Poster # F-055. ASM 2008.
 
 
Shepard JR, Addison RM, Alexander BD, Della-Latta P, Gherna M, Haase G, Hall G, Johnson JK, Merz WG, Peltroche-Llacsahuanga H, Stender H, Venezia RA, Wilson D, Procop GW, Wu F, Fiandaca MJ.  
Multicenter evaluation of the Candida albicans/Candida glabrata peptide nucleic acid fluorescent in situ hybridization method for simultaneous dual-color identification of C. albicans and C. glabrata directly from blood culture bottles. J Clin Microbiol. 2008 Jan;46(1):50-5. Epub 2007 Oct 31.
 
Shepard JR, Merz WG, Gherna M, Hall G, Wilson D, Procop G, Fiandaca MJ. 
Evaluation of Yeast Traffic Light PNA FISH for detection of High Prevalence Candida Species. Poster # F-027. ASM 2007.
 
Wu F, Whittier S, Della-Latta P. 
Tri-color PNA FISH Detection of Five Candida Species Directly from Positive Blood Culture Bottles. Poster # P-1419. ECCMID 2007. 
 
Novak-Weekley SM, LaForga M, Carey L, Frazier L. 
Validation of S. aureus PNA FISH, E. faecalis PNA FISH & C. albicans PNA FISH for Rapid Identification of Positive Blood Culture Bottles. Poster # C-007. ASM 2006. 
 
Fiandaca M, Shepard J, Strauss S, Casiano-Colon A, Romansky S, Hulbert B, Schultz S, Kuusela P, Ristow K, Janda W, Cioffi F, Danielson N, Harkins T, Johansen J. 
A Single-Blinded Multicenter Evaluation of Three PNA FISH Kits for Rapid Species Identification Directly from Blood Culture Bottles. Poster # C-268. ASM 2006. 
 
Wilson DA, Joyce MJ, Hall LS, Reller LB, Roberts GD, Hall GS, Alexander BD, Procop GW. 
Multicenter evaluation of a Candida albicans peptide nucleic acid fluorescent in situ hybridization probe for characterization of yeast isolates from blood cultures. J Clin Microbiol. 2005 Jun;43(6):2909-12. 
 
Rausei V, Youssef E, Morgan M. 
Direct identification of Staphylococcus aureus, Enterococcus faecalis, and Candida albicans from blood cultures using fluorescence in situ hybridization with nucleic acid probes. Poster # V-002. ASM 2004.
 
Rigby S, Procop GW, Haase G, Wilson D, Hall G, Kurtzman C, Oliveira K, Von Oy S, Hyldig-Nielsen JJ, Coull J, Stender H. 
Fluorescence in situ hybridization with peptide nucleic acid probes for rapid identification of Candida albicans directly from blood culture bottles. J Clin Microbiol. 2002 Jun;40(6):2182-6.
 
Oliveira K, Haase G, Kurtzman C, Hyldig-Nielsen JJ, Stender H. 
Differentiation of Candida albicans and Candida dubliniensis by fluorescent in situ hybridization with peptide nucleic acid probes. J Clin Microbiol. 2001 Nov;39(11):4138-41.